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. 2017 Dec 4;6:e24192. doi: 10.7554/eLife.24192

Figure 5. Shisa7 has no effect on short-term plasticity.

(a) Superimposed example traces of whole-cell recordings voltage clamped at –70 mV from CA1 pyramidal neurons of Shisa7 KO animals (red) and WT littermates (grey) in response to 50 Hz stimulation of synaptic inputs from Schaffer collateral fibers. (b) Paired pulse ratio is not affected in Shisa7 KO animals, as exemplified by the 20 Hz data (WT: 1.04 ± 0.02; Shisa7 KO: 1.07 ± 0.03; p=0.439). (c–e) Pulse ratios of electrically-evoked EPSCs from CA1 pyramidal neurons (at –70 mV) of Shisa7 KO animals and WT littermates at 5 Hz (c), 20 Hz (d) and 50 Hz (e). At 5, 20 and 50 Hz, a clear desensitization was observed (factorial repeated measure ANOVA, time: F(2.97,35.65) = 18.21, p<0.001; F(1.55,43.39) = 35.55, p<0.001; F(1.69,21.93) = 35.529, p<0.001, respectively). At none of these frequencies there was a genotype effect or genotype x time interaction (all p>0.3). Cell numbers (n) used are indicated.

Figure 5.

Figure 5—figure supplement 1. Similar AMPAR and NMDAR amplitudes in Shisa7 KO mice.

Figure 5—figure supplement 1.

(a,b) Correlation analysis of stimulus current injected vs. EPSP amplitude (Ln-transformed data) for AMPAR (a; nA) and NMDAR (b; pA) obtained showed no difference for genotype (a, WT n = 56 data points; Shisa7 KO n = 99 data points; (b) WT n = 23 data points; Shisa7 KO n = 33 data points) by linear regression. (c) The averaged ratio of the median amplitude of AMPAR eEPSCs and the median amplitude of NMDAR eEPSCs at each stimulation current was similar in Shisa7 KO and WT slices. Individual ratios are shown. (d,e) Shisa7 KO animals show a faster rise time (WT: 2.80 ± 0.26 ms; Shisa7 KO: 2.00 ± 0.13 ms; unpaired t-test p=0.032) and a faster deactivation time (WT: 16.9 ± 1.4 ms; Shisa7 KO: 12.0 ± 0.9 ms; unpaired t-test p=0.006) for AMPAR eEPSCs (d). There was no significant genotype difference for rise time (WT: 5.0 ± 0.45 ms; Shisa7 KO: 7.0 ± 0.40 ms; unpaired t-test p=0.088) or deactivation time (WT: 46.9 ± 11.8 ms; Shisa7 KO: 51.9.0 ± 5.6 ms; unpaired t-test p=0.686) (e).