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. 2017 May 12;45(13):7736–7750. doi: 10.1093/nar/gkx379

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Mutational analysis of the zinc finger domain present in SPMMV P1. (A) 35S:P1-C85A, 35S:P1-C88A, 35S:P1-C91A, 35S:P1-C103A and 35S:P1-C106A respectively were coinfiltrated with 35S:mGFP4. Photos were taken under UV light at 3 days post agroinfiltration. (B) GFP mRNA was detected by Northern blotting. Extracts of infiltrated leaves were subjected to SDS-PAGE followed by immunoblotting with the anti-GFP antibody. Flag-tagged P1 proteins were detected by Western blotting using the M2-anti Flag antibody. Ponceau staining shows equal loading of proteins in both Western blots. Top panel, mean (n = 3) relative mGFP4 transcript level and ±SD (lane 3 = 1.0 for mGFP4 transcript). One representative blot from three biological replicates is shown.