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. 2017 Jun 20;45(14):e137. doi: 10.1093/nar/gkx542

Figure 4.

Figure 4.

Three-plex Simoa assay for let-7a, let-7b and let-7c and cross-reactivity profiles. (A) Target, capture, and detection probe sequences used in this assay, and multiplex assay results for the direct detection of three different miRNAs simultaneously. (B–D) Cross-reactivity of the multiplex assay in the presence of (B) only let-7a, (C) only let-7b and (D) only let-7c. (E) Detection of let-7a, let-7b and let-7c in 12 samples using the three-plex Simoa assay. Actual spike-in concentrations for each of let-7a, let-7b and let-7c in the samples (S1–S12) are given in the table, and measured concentrations (after compensating for off-target signal as shown in Supplementary Figure S3 and Supplementary Table S3) are indicated in the plot.