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Pcf11 has two zinc-binding domains. Deconvoluted ESI mass spectra obtained from (A) Pcf11 (403–626) and (B) Pcf11 (530–626) constructs under denaturing (upper panels) and non-denaturing (lower panels) conditions. Mass differences between denaturing and non-denaturing spectra respectively correspond to the binding of two (+130.5Da) and one (+65.7Da) zinc atoms.
