Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 29;45(17):10115–10131. doi: 10.1093/nar/gkx674

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 9. — The zinc-binding region can bind RNA in the absence of Clp1. (A) Equal amounts (600 ng) of recombinant proteins were loaded on a 12% SDS-polyacrylamide gel and stained with Coomassie. Lane 1: full-length Rna15; lane 2: Pcf11^[403-626]-Clp1; lane 3: Pcf11^[403-626]; lane 4: Pcf11-18^[403–626] mutant (C⁴²¹S/C⁴²⁴S); lane 5: Pcf11-19^[403–626] mutant (C⁵⁶⁴S/C⁵⁶⁷S). (B) Equal amounts (100 ng) of recombinant proteins as described in (A) were incubated in the presence of ³²P-labeled CYC1 transcript and subjected to UV cross-linking, in the absence (left panel) or presence of an excess of competitor yeast total RNA (5 μg; right panel). The position of the different polypeptides is indicated.