Figure 5.

Cmr1α-M1 does not impact the massive DNA cleavage of Cmr-α complex. Twenty-five nM labeled S10 ssDNA substrate were incubated with 50 nM Cmr-α/400 nM Cmr-α1M1 and 200 nM SS1 RNA, in the presence or absence of 2.5 μM of unlabeled S10 ssDNA as indicated. Reaction was conducted for 60 min during which samples were taken at 5, 30 and 60 min and analyzed by denaturing PAGE.