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. Author manuscript; available in PMC: 2018 Jun 21.
Published in final edited form as: ACS Chem Neurosci. 2017 Feb 15;8(6):1232–1241. doi: 10.1021/acschemneuro.6b00386

Figure 3. Immobilized human rCD63 is able to bind fibrillar Aβ in an in vitro binding assay.

Figure 3

Assay parameters were optimized to achieve best possible signal to noise ratio. (A-D) Curves from checkerboard titrations to determine optimal concentrations for rCD36 (A), fibrillar Aβ (B), anti-Aβ primary antibody (C) and secondary anti-species conjugated antibody (D). (E) Dose response graph of the Ursolic acid (UA) positive control. (F) Optimization of time of addition of inhibitors in the assay. Condition A: the addition of UA and fAβ 1-42 at the same time; Condition B: UA added 30 minutes before and removed just before adding the fAβ 1-42 and; Condition C: UA added 30 minutes before fAβ 1-42 addition. (G) Sensitivity of the assay to DMSO. Results represent means ± S.D. from samples assayed in duplicate.