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. 2017 Aug 2;45(17):10168–10177. doi: 10.1093/nar/gkx694

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Direct comparisons of rates of RRF/EF-G-induced PoTC (0.2 μM) breakdown on rapid mixing with 3 μM EF-G·GTP and 2 μM RRF. (A) PoTC-^thG, as measured by the decrease in mRNA-^thG anisotropy (blue line), tRNA^Phe(prf) anisotropy (black line) or light scattering (red line). (B) PoTC-SD-F7, tRNA^Phe(prf) anisotropy (black line) or light scattering (red line); PoTC-SD-F7-fl, mRNA-^thG anisotropy (blue line). Initial values of mRNA and tRNA anisotropy are offset by +20 and +10%, respectively, for clarity.