Figure 7.

PCR and Sanger sequencing results for four fusion transcripts with low number of FESRs from ChimeRScope predictions. For each fusion transcript track, the left panel is the PCR panel and the right panel displays the predicted fusion sequence and the primer binding site, along with the Sanger sequencing chromatogram. Specifically, each PCR image has four lanes for a 100-bp ladder marker, the fusion transcript amplicon with the band of the matched product pointed by a red arrow, the positive control (actin beta, or ACTB) and negative control (water). The right panel shows the name of the fusion partners, the predicted fusion junction sequence (100 bp upstream and downstream, separated by the wildcard ‘N’), the binding sites of the primer pair used in the PCR panel, the chromatogram for the highlighted region (mostly the fusion junction, if applicable). The PCR experiments and the Sanger sequencing results confirmed the existences of these four genes in the NK cell lines. We were unable to resolve the fusion junctions for RPL14&SRP14 and LRRC37A3&NSF due to the poor Sanger sequencing data quality. Therefore, the exact fusion junctions of these two fusions were not marked in the chromatograms.