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. 2017 May 27;45(12):7226–7236. doi: 10.1093/nar/gkx223

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — SE analyses of representative AGT-pUC19 DNA mixtures. Samples were centrifuged to equilibrium at 3000 rpm and 4°C, as described in ‘Materials and Methods’ section. Equation (1) was used to fit radial absorbance distributions for samples containing ∼11 nM DNA and ∼8.5 μM AGT. Samples contained DNAs of ΔLk = −1 (), of ΔLk = −2 (△) and ΔLk = −5 (♦). Absorbance values were offset vertically to improve visual clarity. The smooth curves correspond to fits of Equation (1) to these data. Small, symmetrically-distributed residuals (upper panels) indicate that the two-species model represented by Equation (1) was consistent with the mass distributions of DNA in these samples.

Inline graphic — SE analyses of representative AGT-pUC19 DNA mixtures. Samples were centrifuged to equilibrium at 3000 rpm and 4°C, as described in ‘Materials and Methods’ section. Equation (1) was used to fit radial absorbance distributions for samples containing ∼11 nM DNA and ∼8.5 μM AGT. Samples contained DNAs of ΔLk = −1 (), of ΔLk = −2 (△) and ΔLk = −5 (♦). Absorbance values were offset vertically to improve visual clarity. The smooth curves correspond to fits of Equation (1) to these data. Small, symmetrically-distributed residuals (upper panels) indicate that the two-species model represented by Equation (1) was consistent with the mass distributions of DNA in these samples.