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Dependence of saturating stoichiometry on linking difference. Samples contained individually purified pUC19 topoisomers (8–12 nM) and 24 μM AGT in buffer containing 10 mM Tris (pH 7.6), 1 mM DTT, 1 mM EDTA, 100 mM NaCl. Stoichiometries were inferred from the weight-average reduced molecular weights of AGT–DNA complexes, measured at SE, as described in the ‘Materials and Methods’ section.
