Table 2. Binding parametersa.
DNA | K ave (l/mol) | ωave | s (bp/AGT or nt/AGT) |
---|---|---|---|
Single-stranded 13-mer | 18 000 ± 1200 | 33.9 ± 2.7 | 4.3 ± 0.04 |
Double-stranded 13-mer | 22 900 ± 1800 | 27.1 ± 2.7 | 4.18 ± 0.06 |
Single-stranded 16-mer | 18 800 ± 3300 | 91.0 ± 16.2 | 4.01 ± 0.02 |
Double-stranded 16-mer | 23 700 ± 6200 | 102.6 ± 28.6 | 3.94 ± 0.06 |
aSamples equilibrated at 20 ± 0.1°C in 10 mM Tris (pH 7.6), 1 mM DTT, 100 mM KCl, 5% glycerol.