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. 2017 Aug 23;45(19):11144–11158. doi: 10.1093/nar/gkx737

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Identification of antisense-regulated genes using whole colony fluorescence measurements. (A) The antisense library contains strains where 188 genes were seamlessly tagged with either sfGFP alone (wt), sfGFP with a unidirectional terminator (PHO5_T), or a scrambled version of PHO5_T (PHO5_T:scr). See Huber et al. (40) for additional details. (B) Whole colony fluorescence intensity ratios of PHO5_T:scr/PHO5_T strains were determined for all strains of the antisense library grown under the three growth conditions indicated. Significantly regulated genes (FDR < 10%) are highlighted along with the respective growth condition (enlarged symbols). Grey dots indicate genes that did not meet some selection criteria. The inset shows the data for the Sps100-sfGFP. (C) Bar plots of the antisense effects for all significantly regulated genes found in (B) for all three conditions tested. The Venn diagram shows the overlap of regulated genes between the three growth conditions.