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. 2017 Aug 23;45(19):11144–11158. doi: 10.1093/nar/gkx737

Figure 2.

Figure 2.

Protein, sense and antisense RNA levels of SPS100 are correlated and the antisense effect is preserved in sporulation. (A) Representative immunoblot and quantification of three biological replicates of Sps100-sfGFP protein levels in Sps100-sfGFP wt, PHO5T and PHO5T:scr strains after growth under starvation conditions (SC, 0.1% glucose, 30°C). (B) Plots showing RT-qPCR of sense and antisense transcripts of SPS100-GFP in the cells from (A). Error bars indicate standard deviation of three biological replicates. (C) Sporulation and starvation time course measurements using a diploid sps100Δ/SPS100-sfGFP hybrid with wt, PHO5T and PHO5T:scr constructed by mating of the haploid SPS100-sfGFP strains (in the BY4741 background) with a sps100Δ strain (in the well-sporulating SK1 background). Fluorescence of sfGFP was quantified by flow cytometry. (D) Representative images showing the spore-autonomous localization of Sps100-sfGFP after 24 h into sporulation.