Figure 7.
Long mRNA isoform of SPS100 is more stable than the short one. Northern blots of a thiolutin chase experiment in the SPS100-PHO5T strain. Samples were taken for RNA extraction in indicated time intervals after thiolutin addition to the media. The upper panel is the blot probed with probe A (see Figure 6), the long and short isoforms are labelled with arrows. The lower panel is the loading control (RNA gel stained with ethidium bromide). Quantification of lower and upper bands from the Northern blots and from another independent replicate is shown on the plot below. Intensity values were normalized to the time point ‘0’, errors bars indicate standard deviations.