Fig. 4.
Effects of drinking hot spring water on the microscopic morphology and the IHI in the stomachs of mice with ethanol-induced gastric mucosal injury. (A) Histological appearances of the stomachs of mice in different groups: (a) DW group; (b) DW + 30% EtOH group; (c) DW + 60% EtOH group; (d) DW + 100% EtOH group; (e) spring water with Rn group; (f) spring water with Rn + 30% EtOH group; (g) spring water with Rn + 60% EtOH group; (h) spring water with Rn + 100% EtOH group; (i) spring water without Rn group; (j) spring water without Rn + 30% EtOH group; (k) spring water without Rn + 60% EtOH group; and (l) spring water without Rn + 100% EtOH group. Scale bar = 100 μm. All samples were stained with HE. The black arrow in (d) indicates typical injury areas. (B) IHI scores. Each value represents a mean ± SEM. The number of mice per experimental point was eight. **P < 0.01, DW with ethanol vs DW with saline. #P < 0.05, Rn-containing hot spring water (spring water with Rn) vs DW with ethanol.