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. 2017 Jun 5;45(14):8484–8492. doi: 10.1093/nar/gkx501

Figure 1.

Figure 1.

Codon usage affects translation elongation rate in S2 cell-free extract. (A) Real-time measurement of firefly luciferase activity using S2 cell-free translation system and the indicated Luc mRNA templates. TFA values are indicated by arrows. Luciferase signals were normalized to the signal level of the OPT mRNA at 21 min. Error bars represent standard deviations of three replicates. (B) Quantification of the TFA values of OPT and dOPT Luc mRNAs. ***P < 0.001. (C) Western blot analysis using luciferase antibody showing the production of the full length luciferase (indicated by the arrow) in the in vitro translation assays. The asterisk indicates the non-specific bands recognized by the luciferase antibody. NC: Negative Control. (D) Real-time measurement of firefly luciferase activity using the S2 cell-free translation system and the indicated Luc mRNA templates. mRNA concentrations are adjusted to produce similar activity levels. Error bars represent standard deviations of three replicates. (E) Quantification of the TFA values of the indicated Luc mRNAs. *P < 0.05, **P < 0.01, ***P < 0.001.