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. 2017 Jun 5;45(14):8484–8492. doi: 10.1093/nar/gkx501

Figure 3.

Figure 3.

Codon usage affects trypsin sensitivity of luciferase protein produced in vitro and in S2 cells. (A) Limited trypsin digestion assay for the full-length OPT and dOPT luciferase proteins translated by the S2 cell-free system for 20 min. A representative western blot was shown in the upper panel. The lower panel shows the densitometric analysis results of LUC levels from independent experiments. **P < 0.01, ***P <0.001. n = 5 for OPT and n = 4 for dOPT mRNAs. Area under curve (AUCs) for OPT and dOPT samples are 860.1 ± 42 and 1195 ± 62.64, respectively. (B) Limited trypsin digestion assay for luciferase protein produced in S2 cells after transfecting the OPT or dOPT expressing construct. A representative western blot was shown in the upper panel. The lower panel shows the densitometric analysis results of LUC levels from three independent experiments. *P < 0.05, **P < 0.01. AUCs for OPT and dOPT samples are 710.7 ± 13.73 and 927.8 ± 38.47, respectively.