Table 1. The high resolution X-ray structure of Fox-1 RRM.
| Fox-1 RRM | |
|---|---|
| Data collections | |
| Beam line | Pilatus 6M X06SA at SLS |
| Wavelength (Å) | 0.999987 |
| Detector distance (mm) | 300 |
| Space group | C121 |
| Unit cell parameters (Å, °) | a = 68.98, b = 77.62, c = 106.39, α = γ = 90, β = 93.99 |
| No. of molecules per asymmetric unit | 6 |
| No. of measured reflectionsa | 18 4971 (26 029) |
| No. of unique reflectionsa | 50 871 (7510) |
| Redundancya | 3.6 (3.5) |
| Completenessa (%) | 98.0 (97.2) |
| Resolution rangea (Å) | 45.41–1.80 (1.90–1.80) |
| R merge a,b (%) | 6.1 (40.9) |
| R meas a,c (%) | 7.2 (48.3) |
| I/σ(I)a | 13.4 (4.0) |
| Refinement | |
| Resolution range (Å) | 45.41–1.80 |
| R work d/Rfreee (%) | 19.4/22.7 |
| No. of residues | |
| Fox-1 RRM | 484 |
| Sulfate | 7 |
| PEG | 1 |
| MES | 4 |
| Water | 331 |
| RMS(bonds) (Å) | 0.008 |
| RMS(angles) (°) | 1.112 |
| Ramachandran Most favoredf (%) | 98.7 |
| Ramachandran Allowedf (%) | 1.3 |
| Ramachandran Outliersf (%) | 0 |
| Average B-factor (Å2) | |
| Fox-1 RRM | 25.4 |
| solvent | 33.2 |
| PDB code | 4zka |
aValues in parentheses are for the highest resolution shell.
b
c
, where <I>h is the mean intensity of symmetry-equivalent reflections and m is multiplicity.
d
, where F0 and Fc are observed and calculated structure factor amplitudes.
e R free calculated for 5% of randomly chosen reflections that were excluded from the refinement.
fRamachandran Plot, as defined by the program PROCHECK (67).