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. 2017 Dec 20;12(12):e0188766. doi: 10.1371/journal.pone.0188766

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© 2017 Stempien et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (A) Laccase activity. Oxidation of ABTS in the radical cation ABTS^.+ was measured with total secreted proteins from N. parvum Bourgogne S-116, N. parvum Bt-67 and D. seriata 98.1. Total proteins were isolated from culture filtrates of fungi grown in malt medium (Malt), malt medium supplemented with V. vinifera sawdust (Malt + W), Erickson and Peterson medium (EP) and Erickson and Peterson medium supplemented with V. vinifera sawdust (EP + W). (B) Manganese peroxidase activity. Oxidation coupling of MBTH/DMAB was measured in presence of H₂O₂ and Mn²⁺. Total proteins were isolated from culture filtrates of fungi grown in liquid malt medium (Malt), malt medium supplemented with V. vinifera sawdust (Malt + W), Erickson and Peterson medium (EP) and Erickson and Peterson medium supplemented with V. vinifera sawdust (EP + W). Values are means and SD of three biological replicates, each calculated from the mean of two technical replicates. Means with a same letter are not significantly different at p≤0,05 (Tukey Contrasts).