Fig 7. Effects of leucine or lysine deficient media on proteolytic molecules gene expression in gilthead sea bream cultured myocytes.

Quantitative gene expression of (A) cathepsins (ctsda, ctsdb, ctsb, ctsl), (B) calpains (capn1, capn2, capn3, capns1a (s1a), capns1b (s1b)) and (C), UbP members (mafbx, murf1, n3, ub) relative to the geometric mean of ef1α and rps18 in myocytes at days 2, 4 or 8 of culture after incubation from day 1 (for samples at days 2 and 4) or day 7 (for samples at day 8) with a growth medium deficient in leucine (open bars) or lysine (filled bars). Data are shown as fold change relative to control condition (growth medium without AA deficiencies), represented by the dotted line. Mean ± SEM (n = 3–4 independent cultures). Asterisks indicate significant differences compared to the control at each time (*: p<0.05; **: p<0.01; ***: p<0.001).