Fig. 1.

Cisplatin was induced up to cell death and kidney injury biomarkers in HK2 cells. Cell viability of HK-2 cells was treated with cisplatin low dose-long time manner for 24 h (a), and high dose-short time treatments for 2 to 6 h (b). Release of LDH (c) and HMGB1 (d) were treated with cisplatin (400 μM) for 2 to 6 h. Secretion levels of NGAL (e), KIM-1 (f, g) and HMGB1 (h) for 6 to 48 h. The statistical significance (*p < 0.05, **p < 0.01 and ***p < 0.001) of the observed differences between cisplatin treated and control groups were tested by t-test