Table 2. Plasma amino acids (AA) concentrations at 12 hour after rumen-protected methionine (RPM) feeding.
| Plasma AA concentration, μM1,2,3 | |||
|---|---|---|---|
| AA | CON (n = 4)4 | RPM (n = 6)4 | P-value5 |
| Alanine | 305.7 ± 22.8 | 315.6 ± 13.5 | 0.70 |
| Aspartic acid | 6.6 ± 0.7 | 6.1 ± 0.5 | 0.51 |
| Glutamic acid | 63.7 ± 4.9 | 68.4 ± 4.9 | 0.53 |
| Glycine | 286.0 ± 16.4 | 302.9 ± 18.0 | 0.53 |
| Histidine | 47.6 ± 4.1 | 42.4 ± 1.1 | 0.29 |
| Isoleucine | 123.3 ±3.7 | 121.1 ± 5.6 | 0.78 |
| Leucine | 123.0 ± 9.2 | 123.6 ± 5.2 | 0.95 |
| Lysine | 69.0 ± 9.2 | 68.1 ± 2.7 | 0.93 |
| Methionine | 23.7 ± 0.3 | 42.5 ± 2.8 | 0.001 |
| Phenylalanine | 38.7 ± 1.7 | 40.2 ± 1.1 | 0.46 |
| Proline | 99.3 ± 3.9 | 103.3 ± 4.7 | 0.56 |
| Serine | 130.5 ± 9.9 | 130.8 ± 3.4 | 0.98 |
| Threonine | 127.9 ± 9.9 | 143.0 ± 5.8 | 0.19 |
| Tyrosine | 43.6 ± 2.9 | 47.1 ± 1.5 | 0.26 |
| Valine | 260.8 ± 13.0 | 260.0 ± 10.2 | 0.96 |
1Treatments: Control (CON) = feeding with 60 g of dried distillers grain (1.87% Met of MP); and Rumen-protected methionine (RPM) = 21.2 g of rumen-protected methionine (Smartamine M, 2.34% Met of MP) + 38.8 g of dried distillers grain.
2All AA considered essential and nonessential. Concentrations of arginine, cysteine tryptophan, glutamine, and asparagine, were not obtained.
3Data are presented as mean ± SEM.
4CON = 4 composites (16 cows, 8 primiparous and 8 multiparous); RPM = 6 composites (24 cows, 12 primiparous and 12 multiparous).
5P-values for comparisons between CON and RPM