. 2017 Dec 20;12(12):e0189117. doi: 10.1371/journal.pone.0189117

Table 4. Determination of synchronization based on progesterone (P4) concentrations.

Cows were considered to not be synchronized if they had P4 < 1.0 ng/mL on Day of PGF_2α, P4 > 0.7 ng/mL on Day of last GnRH, or P4 < 0.5 ng/mL on Day 5 after AI.

	Cows		Pregnancies/AI (P/AI)
Day of	Not Synch¹	Synch²	Not Synch¹	Synch²	P³
PGF_2α (< 1.0 ng/ml)	2.9%	97.1%	11.1%	57.5%	0.01
PGF_2α (< 1.0 ng/ml)	(9/308)	(299/308)	(1/9)	(172/299)	0.01
Last GnRH (> 0.7 ng/ml)	3.2%	96.8%	10.0%	57.9%	0.008
Last GnRH (> 0.7 ng/ml)	(10/309)	(299/309)	(1/10)	(173/299)	0.008
Day 5 after AI (< 0.5 ng/ml)	2.0%	96.8%	25.0%	57.8%	0.05
Day 5 after AI (< 0.5 ng/ml)	(8/304)	(296/304)	(2/8)	(171/296)	0.05
Overall⁴	7.8%	92.2%	12.5%	60.0%	< 0.001
Overall⁴	(24/309)	(285/309)	(3/24)	(171/285)	< 0.001

¹Not Synch = cows not synchronized.

²Synch = cows synchronized.

³P–value for comparisons of P/AI between not synchronized cows and synchronized cows.

⁴If a cow was not synchronized by any of the 3 criteria then it was not included in the overall value.