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. 2017 Dec 20;12(12):e0189117. doi: 10.1371/journal.pone.0189117

Table 5. Effect of rumen-protected methionine (RPM) feeding on Pregnancy-specific protein (PSPB) and progesterone (P4) concentrations on Day 28 in cows not pregnant, pregnant on Days 28, 32, and 61, or pregnant on Day 28 that subsequently lost their pregnancy.

Pregnancy status
Item/Treatment1,2 Not Pregnant Pregnant on Day 28, 32 and 61 Pregnant on Day 28 but not on Day 61
PSPB (Day 28, ng/ml)
CON 0.32 ± 0.04 (48) 3.27 ± 0.12 (72) 2.71 ± 0.28 (15)
RPM 0.32 ± 0.03 (48) 3.05 ± 0.09 (80) 2.54 ± 0.33 (9)
P-value3 0.90 0.22 0.55
Overall4 0.32 ± 0.02 (96)a 3.15 ± 0.07 (152)b 2.65 ± 0.20 (24)c
P4 (Day 28, ng/ml)
CON 2.0 ± 0.3 (47) 8.3 ± 0.3 (75) 8.1 ± 1.2 (15)
RPM 2.5 ± 0.5 (48) 8.0 ± 0.4 (81) 6.0 ± 0.9 (9)
P-value3 0.70 0.83 0.21
Overall4 2.3 ± 0.3 (95)a 8.1 ± 0.2 (156)b 7.3 ± 0.8 (24)b*

1Treatments: Control (CON) = feeding with 60 g of dried distillers grain (1.87% Met of MP); and Rumen-protected methionine (RPM) = 21.2 g of rumen-protected methionine (Smartamine M, 2.34% MET of MP) + 38.8 g of dried distillers grain.

2Data are presented as mean ± SEM.

3P–values for comparisons between CON and RPM.

4Mean ± SEM values with different superscripts in the same row are different (P < 0.001) or similar superscripts with an asterisk (*) tended to be different (P = 0.06).