Fig. 2.

Exon-intron structure, conserved motif and conserved domain analysis of GhNPC genes. a. Phylogenetic tree of GhNPCs. Phylogenetic tree was generated using MEGA7 by the Maximum Likelihood method based on the protein sequences of GhNPCs. b. Exon-intron structures. Exons and introns of G. hirsutum are shown as yellow rounded rectangles and thin lines, respectively. c. Conserved motif analysis. Twenty motifs were identified. Different color boxes represent different types of motifs. Sequence information of twenty motifs was shown in Additional file 8: Table S5. d. Conserved domain analysis. The conserved domains of NPC protein were examined by NCBI Conserved Domain search and SMART, and were located with HMMSCAN