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. 2017 Dec 20;14:237. doi: 10.1186/s12985-017-0905-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Islet cells were cultured overnight and then dispersed enzymatically. Cells were then stained for viability, surface CD81 (or IgG₁ isotype), and intracellular insulin. Dot plots are gated on live cells with high FSC/SSC properties typical of endocrine cells. Insulin negative cells are mostly alpha cells