Immunohistochemical analyses for cytokines in duodenum Peyer’s patches after TBLF treatment. A) IL-6 analysis, B) Nfκ-B analysis, C) TNF-α analysis. All immunohistochemical analyses were performed at 10X resolution. Red arrows show positive signals; a) smooth muscle, b) primary follicle, c) lamina propria, d) M cells. D) The Peyer’s patches in the treated rats showed more lymphoid follicles (white arrows) and a thinner appearance than in control intestines. Photographs were taken using a Carl Zeiss macro 100 lens with extension tubes. There were analysed 40 slices per group from at least 3 different animals.