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. Author manuscript; available in PMC: 2019 Jan 5.
Published in final edited form as: J Mol Biol. 2017 Nov 11;430(1):33–40. doi: 10.1016/j.jmb.2017.11.002

Fig. 1.

Fig. 1

Tryptophan fluorescence emission spectra (280 nm excitation) of wild-type HdeA (a) and HdeA E37Q (b) as a function of pH. The insets show the fluorescence emission at 335 nm as a function of pH. HdeA contains two tryptophans: Trp82 (in close proximity to Glu37) and Trp16 (more distant from Glu37). Fit parameters for WT HdeA (a): n1 = 2.04, pKa1=2.81, n2=1.09, pKa2=5.59. Fit parameters for HdeA E37Q (b) n = 2.23, pKa = 3.14.