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Subtype distribution of anti-nicotine IgGs. The titers of anti-nicotine IgG subtypes on day 40, including (A) IgG1, (B) IgG2a, (C) IgG2b, and (D) IgG3, were assayed. (E) shows the relative percentages of subtype anti-nicotine IgGs. Significantly different compared to NanoNicVac with no adjuvant: # p < 0.05, ## p < 0.01, and ### p < 0.001. Significantly different: ** p < 0.01, *** p < 0.001.
