Fig. 4.

2D ¹H-¹³C HSQC spectra of the U-¹⁵N,¹³C-labeled Abl1b RM acquired on an 850 MHz spectrometer. (A) original Bruker pulse sequence (hsqcetgpsisp2.2, Fig. S3A); (B) replacing all ¹H hard π pulses with G5; (C) replacing all ¹H and ¹³C hard pulses with G5. (D) Overlay of 1D projections of (A) and (C). The numbers in (B) and (C) represent the ratios of the signal intensities of (B) and (A), and (C) and (A), respectively. Acquisition parameters: d1 = 1 sec, ns = 16 (number of scans), TD = 2k×256, p1 = 11.3 μs (length of ¹H π/2 hard pulse), p3 = 11.2 μs (length of ¹³C π/2 hard pulse), the offsets of ¹H and ¹³C are 4.7 and 75.0 ppm, respectively. The spectral width of the ¹³C dimension was 165 ppm.