Fig. 2.

Snail represses 4E-BP1 expression at both the protein and mRNA levels. a HEK293, T47D, MCF7, and HCT116 cells with stable expression of Snail or vector control were analyzed by western blotting for the indicated proteins. b mRNA expression of the indicated genes was analyzed by quantitative RT-PCR in T47D, MCF7, and HCT116 cells with stable expression of Snail or vector control. The indicated gene expression was normalized against GAPDH and presented as a percentage of the expression level found in vector control cells. c HCT116, MDA-231, and SUM149 cells with stable expression of two different sets of Snail shRNAs (ShSnail_1 and ShSnail_2) or control shRNA (ShCtrl) were analyzed by western blotting for the indicated proteins. d mRNA expression of the indicated genes was analyzed by quantitative RT-PCR in HCT116, MDA-231, and SUM149 cells with stable expression of ShSnail_1, ShSnail_2, or ShCtrl. The indicated gene expression was normalized against GAPDH and presented as a fold increase over the expression level found in ShCtrl cells. All graphic data are presented as mean ± SEM (n = 3 technical replicates per condition). *P < 0.001 for Snail vs. vector or ShSnail vs. ShCtrl using Student's t-test