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. 2017 Dec 20;7:17943. doi: 10.1038/s41598-017-18190-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Withaferin A treatment inhibits HCC growth in nude mice. HepG2 cells-derived tumors were developed in nude mice and treated with vehicle or WA. (A) Tumor growth was monitored by measuring the tumor volume for 4.5 weeks (n = 8 mice per group). *p < 0.001, compared with vehicle-treated group. (B) Tumors weight and volume were measured at the end of treatment period. Average tumor weight and representative tumor images are shown here. (C) Tumor lysates were immunoblotted for the expression of cleaved caspase 3, Bax, cleaved PARP and PCNA as indicated. (D) Tumor lysates were immunoblotted for the expression of DR5, phosphorylated and total ERK, ELK1, RSK as indicated. β-Actin antibody was used as loading control. (E) Tumor samples were subjected to immunohistochemical analysis using phospho-ERK1, phospho-ELK1, phospho-RSK and DR5 antibody.