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. 2017 Dec 20;7:17933. doi: 10.1038/s41598-017-18085-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative activities of FSA and EH-CC variants in the presence of Ca²⁺ and Zn²⁺. (a) FSA and its mutants; (b) EH-CC and its mutants. The activity was measured after pre-incubating the starch-free reaction mixtures with 0.5 mM Ca²⁺, 0.01 mM Zn²⁺, or both ions, at 50 °C for 30 min. The activity of FSA in the absence of metal ions and without pre-incubation was set as 100%.