Figure 5.

DSC spectrums of the purified FSA (a) and EH-CC-E204G (b). The scanning profile in the absence of metal ions (black line), in the presence of 0.5 mM Ca²⁺ (blue line), 0.01 mM Zn²⁺ (green line), and both ions (red line). Using the dialysis buffer as the reference, 0.8 mg ml⁻¹ of FSA and EH-CC-204 were scanned from 20 to 90 °C.