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Thermodynamics of metal ions binding.
| Metal ion | Site | Parameter | FSA | EH-CC-E204G |
|---|---|---|---|---|
| Ca2+ | Site 1 | K d (μM) | 746.3 | 653.6 |
| ΔH (kcal/mol) | 1.3 | 1.2 | ||
| Zn2+ | Site 1 | K d (μM) | 1642.0 | 724.6 |
| ΔH (kcal/mol) | −108.3 | −64.2 | ||
| Site 2 | K d (μM) | 2941.2 | 4524.9 | |
| ΔH (kcal/mol) | 185.4 | 6.9 | ||
| Site 3 | K d (μM) | 444.4 | 96.2 | |
| ΔH (kcal/mol) | 242.1 | 67.7 | ||
| Site 4 | K d (μM) | 1422.5 | ||
| ΔH (kcal/mol) | 260.8 | |||
| Site 5 | K d (μM) | 961.5 | 869.6 | |
| ΔH (kcal/mol) | −159.4 | −109.7 |
