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. 2017 Dec 20;7:17949. doi: 10.1038/s41598-017-18186-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Modulation of the major survival/death pathways by anti-cancer drugs after ectopic expression of miR-34a in MM cells. RPMI 8226 cells were transfected with miR-34a or miR-NC and treated with γSI, Sirtinol and ZOL, as described in “Methods”. After 48 hours treatment, cells were collected for Western blotting analysis. Subsequently, the expression of SIRT1, p53(Ack 382), NICD, AKT, p-AKT, ERK1/2, p-ERK1/2, pro-Caspases 7, 3, 8 and 9, and their cleaved fragments, were evaluated after blotting with specific antibodies, as described in “Methods” (a/b). Histograms show the variation of protein levels with respect to total proteins, by Image Lab 5.2.1 ChemiDocTM XRS + (BIORAD). The results are shown as the ratio between the active and non-active forms and compared to control cells levels (c). Each experiment was repeated at least three times and data are shown as mean ± standard deviation (SD). *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.