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. 2017 Dec 20;7:17949. doi: 10.1038/s41598-017-18186-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometric analysis of autophagy. RPMI 8226 cells were transfected with miR-34a or miR-NC and treated for 48 h with γSI, Sirtinol and ZOL, as described in “Methods”. After MDC staining, cells were analysed on the BD Accuri C6™ flow cytometer (a). (b) The histogram shows the relative MFI with respect to control sample. The results are shown as means ± standard deviation (SD) for three independent experiments. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.