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. 2017 Dec 20;7:17885. doi: 10.1038/s41598-017-18273-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Linear plasmid characterization of pEC2474_MCR with closely related plasmid pHNSHP45-2 (KU341381). The grey regions between plasmids indicate nucleotide identity (65–100%) by BLASTN. Gray shades indicate shared regions with high degree of homology. Arrows indicate predicted open reading frames (ORFs) and colored according to their putative functions. Blue arrows indicate replication associated genes. Yellow arrows indicate conjugal transfer-involved genes. Genes associated with plasmid stability are colored by brown. Antimicrobial resistance genes and mobile elements genes were indicated by red and green arrows, respectively. Grey arrows indicate genes for hypothetical proteins as well as proteins with unknown function.