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. 2017 Dec 20;7:17904. doi: 10.1038/s41598-017-18204-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative images of fibronectin immunohistochemical detection in tissue of Balb/c mice from each experimental group. (A) Control, (B) Magh-cit, (C) Rh₂(H₂cit)₄, (D) Magh-Rh₂(H₂cit)₄, (E) Quantification of FN staining. The data represent the average of 10 photos of each group. In Magh-Rh₂(H₂cit)₄ treated animals, the percentage of tissue area occupied by positive cells is significantly reduced compared to the control group mice. (A–D) IHC, DAB chromogen, Mayer’s Hematoxylin counterstain, Original magnification 200x. Data represent mean values ± standard error and different letters indicate statistical difference among treatments (p < 0.05). Scale bars: 50 µm.