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. 2017 Dec 20;7:17904. doi: 10.1038/s41598-017-18204-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of FN and LN proteins expression of Balb/c mice bearing 4T1 breast tumor. The animals were treated with Magh-cit or Rh₂(H₂cit)₄ or Magh-Rh₂(H₂cit)₄. (A) western blotting analysis shows a band of approximately 250 kDa (FN) and 225 kDa (LN) in the total protein extracts from normal and tumor tissues from mice. (B) Quantification of relative protein expression of LN and FN found in western blotting analysis. GAPDH was used as internal control. The healthy and Magh-Rh₂(H₂cit)₄ treated groups showed markedly statistically significant reduction when compared to the control group. The values shown are the mean ± SEM *p < 0.05 and analyzed by an ANOVA; Tukey’s post hoc test.