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. 2017 Dec 20;7:17904. doi: 10.1038/s41598-017-18204-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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mRNA levels of FN1 (A) and LAMA1 (B) were measured subject quantitative reverse transcriptase PCR in breast tumor and health breast tissues. Gene expression in breast tumor tissue treated with Magh-cit, Rh₂(H₂cit)₄ and Magh-Rh₂(H₂cit)₄ was compared with control (non-treated) and health group. The cDNA was prepared and 1.5 µg was used for each RT-PCR reaction. Three independent experiments were average. The values shown are the mean ± SEM of three independent experiments in triplicates, **p < 0.01 and ****p < 0.0001.