Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 20;7:17922. doi: 10.1038/s41598-017-17911-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

High-cholesterol diet causes hypercholesterolemia, increases cholesterol level in brain and disrupts blood-brain barrier (BBB). Control (CON) mice received normal diet and the HCD group received high-cholesterol diet (at 5% w/w mixed with normal diet) for 12 weeks and sacrificed on the last day. (A) Cholesterol level in blood serum (n = 8). (B) Cholesterol level in brain. Brain cholesterol level was assayed histologically in paraformaldehyde fixed tissue sections following Schultz’s method. Photographs are representative sections showing the cortex (CX), striatum (NCP), hippocampus (HP) and substantia nigra (SN) from control and HCD mice. The blue or greenish-blue colour denotes cholesterol. The dark circles denote air bubbles formed as a result of acid (sulphuric acid: acetic acid) reaction. Photographs were taken at 4× magnification. (C) Estimation of brain cholesterol level. Cholesterol level was estimated from tissue homogenates of CX, NCP, HP and SN regions of brain by using kit (n = 6). (D) BBB disruption. Evans Blue dye (EBD) extraversion in brain tissues were analysed for possible disruption of BBB using a Spectrophotometric method (n = 4). The results are mean ± S.E.M. *P ≤ 0.05 or **P ≤ 0.01 as compared to control. Data of cholesterol level in serum (A) and brain EBD were analyzed using an unpaired Student’s t-test (D), and brain cholesterol levels were analyzed using a two-way repeated measure ANOVA (C).