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. 2017 Dec 20;7:17951. doi: 10.1038/s41598-017-18344-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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4-Br-BnIm does not induce Hsp70 in Tg-MYOC^Y437H mice. (a) Representative images depicting Hsp70 levels (red fluorescence), as observed by fluorescent immunostaining and multiphoton microscopy, in the trabecular meshwork (TM) of mouse tissue. TM and ciliary body (CB) are labeled. DAPI is used as a nuclear counterstain (blue). Scale Bar = 50 µm. (b) Quantification of Hsp70 levels normalized to WT vehicle-treated controls. Error bars represent mean ± SEM. Eyes assessed: WT + vehicle (n = 2), WT + 4-Br-BnIm (n = 3), Tg-MYOC^Y437H + vehicle (n = 7), Tg-MYOC^Y437H + 4-Br-BnIm (n = 4). No significant difference was observed between groups as determined by one-way ANOVA analysis, F = 2.8, df = 15. (c) Western Blot analysis and quantitation of Hsp70 levels following vehicle, 17-AAG, and 4-Br-BnIm treatment to HTM cells.