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. 2017 Dec 20;7:17953. doi: 10.1038/s41598-017-18159-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Conversion of arginine¹⁷² to alanine or lysine eliminates agonism of orthosteric agonists but not of DIM. (a) Membranes expressing either GPR84-Cys³⁵²IleGα_i2, Arg¹⁷²Ala GPR84-Cys³⁵²IleGα_i2 or Arg¹⁷²Lys GPR84-Cys³⁵²IleGα_i2 were used in [³⁵S]GTPγS binding studies to assess function of (ai) DIM, (aii), C10, (aiii) embelin and (aiv) compound-1. (b) Similar studies were performed on membrane of cells induced to express FLAG-hGPR84-eYFP (bi), FLAG-Arg¹⁷²Ala hGPR84-eYFP (bii), FLAG-Arg¹⁷⁴Ala hGPR84-eYFP (biii) to assess activity of C10, DIM, embelin and compound-1.