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. 2017 Dec 20;7:17890. doi: 10.1038/s41598-017-17973-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Absence of CycG affects p53 biosensor response to IR-stress in salivary glands. p53R-GFPnls reporter activity was analysed in larval salivary glands from either control, cycG ^HR7 or cycG ^eoC mutants irradiated with 40 Gy. (a) IR-stress results in p53 activation and subsequent expression of nuclear GFP from the p53 biosensor in the control, however to a much lower degree in cycG mutant glands. Size bar represents 100 μm in all panels. (b) Nuclear intensity of GFP signals was recorded using ImageJ by measuring individual nuclei (30–40 per gland; 6–8 glands per genotype). In both cycG mutant alleles nuclear GFP staining is reduced to about one-third of the value measured in the wild type. Significance was tested by ANOVA two-tailed Tukey-Kramer approach (***p < 0.001; ns, not significant).