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. 2017 Dec 20;7:17936. doi: 10.1038/s41598-017-18256-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Decreased SOCE by STIM2-knockdown. The Ca²⁺ in the SR of the STIM2-knockdown myotubes was depleted by the treatment of TG (2.5 μ M) in the absence of extracellular Ca²⁺. Extracellular Ca²⁺ (2 mM) was applied to the myotubes to induce SOCE. (a) A representative trace for each group is shown. The results are summarized as bar graphs for the area under the peaks (b) or the slope at the rising phase of the peaks (c). *Significant difference compared with the untransfected control (P < 0.05). The values are presented as the mean ± S.E. for the number of myotubes shown in the parentheses of Table 4. SOCE was significantly decreased by STIM2-knockdown.