Table 4.
Properties of the STIM2-knockdown mouse primary skeletal myotubes.
| Untransfected | Scrambled siRNA | STIM2-knockdown | ||
|---|---|---|---|---|
| Resting [Ca2+]cytosol, nM | 72.75 ± 9.16 (108) | 72.50 ± 5.02 (116) | 56.17 ± 6.05* (113) | |
| Releasable Ca2+ from the SR | 1.00 ± 0.09 (83) | 0.99 ± 0.08 (88) | 1.30 ± 0.14* (81) | |
| SOCE | Peak area | 1.00 ± 0.11 (76) | 1.01 ± 0.12 (79) | 0.80 ± 0.08* (73) |
| Slope (the rate of the increase) | 1.00 ± 0.05 (20) | 0.97 ± 0.04 (20) | 0.82 ± 0. 04* (20) | |
| KCl response | 1.00 ± 0.08 (74) | 1.03 ± 0.07 (88) | 1.31 ± 0.08* (92) | |
| Caffeine response | 1.00 ± 0.09 (74) | 1.02 ± 0.09 (88) | 1.26 ± 0.11* (92) | |
The values, except for those of the cytosolic Ca2+ levels at rest, were normalized to the mean value of those from the untransfected controls. The values are presented as the mean ± S.E. for the number of myotubes shown in parentheses. *Significant difference compared with the untransfected control (p < 0.05).