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. 2017 Dec 20;7:17923. doi: 10.1038/s41598-017-18054-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TM-replacement did not alter the assembly and viral protein compositions. (A) Structural schematics of influenza hemagglutinin (HA) proteins. The replaced transmembrane (TM) domain is marked grey. (B) Electron images of negatively stained purified H9N2-WT and rH9N2-TM virus particles. Scale bar is 100 nm. (C) SDS-PAGE of viral components of H9N2-WT and H9N2-TM.