Figure 5.
Correction for finite filament length. (A) Shown here is a schematic depiction of GFP-labeled kinesin-1 motors that reach the end of the microtubule and are forced to detach there (scored as “end-event”). (B) Shown here is the simulation of motor proteins landing on 10 filaments, which are picked out of a Schulz distribution with L0. Some motors reach the end and detach prematurely, resulting in underestimation of the run length. Using the Kaplan–Meier estimator (for end-events), a corrected cumulative probability distribution can be calculated and LSF-CDF(free) is used to estimate a corrected run length (the same can be applied to the interaction time). (C) Shown here is experimental data of kinesin-1 run length, where traces were separated in five groups according to the length of their filament (N ≈ 1000). Solid line shows mean run length of combined distribution with bootstrapping error (dashed lines; see Materials and Methods). To see this figure in color, go online.